The Influence of Melanoma Extracellular Vesicles on Benign Melanocytes: A Role for PRAME in Modulation of the Tumor Microenvironment

This study investigates the role of PRAME (Preferentially Expressed Antigen in Melanoma), a cancer-testis antigen, in melanoma progression, particularly its function in melanoma-derived extracellular vesicles (EVs) and its impact on benign primary melanocytes. To investigate the effects of extracellular vesicles from melanoma cells on neighboring benign cells, we treated primary melanocytes with melanoma-derived EVs from A375 cells. We then extracted total RNA and performed RNA sequencing.

本研究探讨了黑色素瘤优先表达抗原（PRAME，Preferentially Expressed Antigen in Melanoma）——一种癌症-睾丸抗原——在黑色素瘤进展中的作用，尤其是其在黑色素瘤来源细胞外囊泡（EVs）中的功能，以及对良性原代黑素细胞的影响。为探究黑色素瘤细胞来源的细胞外囊泡对邻近良性细胞的作用，我们采用A375细胞的黑色素瘤来源细胞外囊泡处理原代黑素细胞。随后我们提取总RNA并开展RNA测序。