Inter-species array between human, rat and mouse

We propose a new microarray method, called an inter-species array, that can measure gene expression levels of multiple species at once. As the array operates simply by changing the probes on an Agilent commercial customized array, and conventional facilities and protocols can be used, the method is cost-efficient. We generated an array for humans, rats and mice that covers 6683 genes. The number of genes is larger than that of previous arrays. We measured the expression of genes in astrocyte cells for humans and rats, and in cortex cells for rats and mice. Single array with multiple species samples.

本研究提出一种新型微阵列（microarray）方法，命名为种间微阵列（inter-species array），可同时测定多个物种的基因表达水平。该方法仅需在安捷伦（Agilent）商用定制化微阵列上更换探针即可运行，且可沿用现有实验设施与实验方案，因此具备较高的成本效益。本研究针对人类、大鼠与小鼠构建了覆盖6683个基因的种间微阵列，其覆盖基因数量优于既往同类微阵列。我们分别针对人类与大鼠的星形胶质细胞（astrocyte cells），以及大鼠与小鼠的皮层细胞（cortex cells）开展了基因表达检测。单张微阵列可兼容多物种样本的检测工作。