Influenza A virus modulation of Streptococcus pneumoniae infection using ex vivo transcriptomics in a human primary lung epithelial cell

Deep sequencing of cDNA from Streptococcus pneumoniae EF3030 bacteria and normalized human broncial epithelial cells (nHBEC), alone, together, and after coincubation with Influenza A virus pH1N1. Overall design: Total RNA was isolated from EF3030 and nHBEC, alone and after co-culture with pH1N1 over time. Ribosomal RNA was depleted and the remaining RNA was reverse-transcribed into a cDNA library for Illumina sequencing. Reads were mapped to the EF3030,and the human genome.

本数据集为肺炎链球菌（Streptococcus pneumoniae）EF3030与标准化人支气管上皮细胞（normalized human bronchial epithelial cells，nHBEC）单独培养、两者共培养，以及二者与甲型流感病毒pH1N1共孵育后的cDNA深度测序数据。实验设计概述：分别从单独培养的EF3030、nHBEC，以及与pH1N1共培养不同时长的样本中提取总RNA；随后去除核糖体RNA，将剩余RNA反转录构建cDNA文库，采用Illumina测序平台进行测序；将所得测序读段分别比对至EF3030基因组与人类基因组。