Circular RNA expression profiling in UVA-treated HaCaT cells

This RNA sequencing dataset analyzes circRNA expression changes in HaCaT keratinocytes after UVA irradiation. Cells were exposed to 5 J/cm2 UVA and profiled using ribosomal RNA-depleted RNA sequencing on an Illumina platform. Differentially expressed circRNAs were identified by computational pipelines. The data provide insights into circRNA regulation during photo-oxidative stress. Overall design: HaCaT cells were cultured in DMEM with 10% FBS and antibiotics. For UVA treatment, cells at 70% confluence were exposed to 5 J/cm2 UVA irradiation using a UV lamp. Control cells were sham irradiated. After 6 hours, total RNA was isolated using TRIzol reagent. Ribosomal RNA was removed and sequencing libraries constructed for Illumina sequencing. Raw reads were aligned to the hg38 human reference genome. Back-spliced junction reads were used to identify and quantify circRNA expression.

本RNA测序数据集旨在分析紫外线A（ultraviolet A，UVA）辐照后人角质形成细胞株HaCaT中环状RNA（circular RNA，circRNA）的表达变化。研究人员将细胞以5 J/cm²的剂量接受UVA辐照，并采用核糖体RNA去除型RNA测序（ribosomal RNA-depleted RNA sequencing）在Illumina测序平台上完成表达谱检测。通过计算分析流程鉴定得到差异表达的环状RNA，本数据集可为光氧化应激（photo-oxidative stress）过程中的环状RNA调控机制研究提供参考。整体实验设计如下：将HaCaT细胞培养于添加10%胎牛血清（fetal bovine serum，FBS）及抗生素的杜氏改良伊格尔培养基（Dulbecco's Modified Eagle Medium，DMEM）中。待细胞汇合度达70%时，对处理组细胞使用紫外灯进行5 J/cm²的UVA辐照，对照组细胞则接受假辐照处理。辐照6小时后，采用TRIzol试剂（TRIzol reagent）提取总RNA，去除核糖体RNA后构建测序文库，用于Illumina测序。将原始测序reads比对至hg38人类参考基因组，通过反向剪接连接reads（back-spliced junction reads）鉴定并定量环状RNA的表达水平。