DESeq2 on RSEM and MultiDEA expressions.

Results of DESeq2 differential expression analysis performed on RSEM and MultiDEA isoform expression estimation. The isoforms are sorted by start position on chr10, and even if RSEM and MultiDEA show some different results, it is possible to deduce that the senescence expression decrease influences both the transcript overlapping SALNR and the ones not overlapping it. Statistically significant count is the number of times that the isoform resulted significantly down-regulated in the 8 tested datasets; XR_007061960 is an additional isoform of HELLS proposed by RefSeq and ENST00000432120 is a lncRNA that overlaps HELLS; a result is considered statistically significant (*) if mean read count >20, absolute log2 Fold Change >1 and adjusted p-value (FDR) (XLSX)

本研究基于RSEM与MultiDEA的转录本（isoform）表达定量结果，开展了DESeq2差异表达分析。所有转录本均按照10号染色体（chr10）上的起始位置进行排序；尽管RSEM与MultiDEA的分析结果存在一定差异，但可推断细胞衰老相关的表达下调同时影响了与SALNR存在重叠的转录本，以及不与之重叠的转录本。统计学显著计数指某转录本在8个测试数据集中共被鉴定为显著下调的次数；XR_007061960是RefSeq注释的HELLS的附加转录本，ENST00000432120则是与HELLS存在重叠的长链非编码RNA（lncRNA）；当满足平均读段计数>20、绝对对数2倍变化（log2 Fold Change）>1且校正P值（错误发现率，FDR）符合要求时，该结果被认定为统计学显著（*），完整数据详见XLSX格式文件。