[Post embryonic development of Chrysomya putoria (Calliphoridae) using horse meat and its importance for forensic entomology and veterinary ]

ABSTRACT Due to medical, veterinary, environmental and forensic importance of the calliphorid Chrysomya putoria (Diptera), the performance presented by immature stages, reared on horse meat, was evaluated. The specimens were placed in acclimatized chambers regulated at 30 ºC, 60 ± 10% RU and 14 hours of photo phase. The larval and pupal stages completed their development in 4, 16 and 4, 12 days, respectively. Post-feeding larvae and aged 24h pupae weight on average 58, 06 and 40, 10 mg, respectively. The emergence rates recorded were 65% under the experimental conditions proposed. As volatile organic compounds are different in the decomposition of one animal to another, this work and others carried out with the same diet are the most appropriate for estimating post-mortem interval involving horses.

摘要：鉴于双翅目（Diptera）丽蝇科（Calliphoridae）的Chrysomya putoria在医学、兽医学、环境科学及法医学领域均具有重要研究价值，本研究对以马肉为食的该蝇未成熟虫态的生长发育表现进行了评估。实验标本被放置于调控参数为30℃、相对湿度60±10%（RU）以及14小时光照周期的人工气候箱中培养。幼虫期与蛹期的发育时长分别为4.16天与4.12天；饱食后幼虫与24小时龄蛹的平均体重依次为58.06 mg与40.10 mg。在本实验设定的条件下，该蝇的羽化率达65%。由于不同动物尸体在分解过程中释放的挥发性有机化合物存在差异，本研究及其他采用相同食源开展的相关研究，在估算以马为受试对象的死后间隔时间（post-mortem interval，PMI）时最为适用。