A time-course transcriptomic study for investigating the genome-wide effects of nitrate on gene expression in mature nodules of Lotus japonicus.

A time-course transcriptomic study was performed on mature nodules of Lotus japonicus MG-20 at 4 weeks post-inoculation (wpi) with Mesorhizobium loti MAFF303099. Plants were exposed to 10 mM KNO3 for six days and then nitrate was removed for five more days. Overall design: Nodule samples were collected daily during nitrate treatment (T0 to T6) or nitrate removal (R1 to R5), each with three biological replicates.

本研究针对接种洛氏根瘤菌（Mesorhizobium loti）MAFF303099后4周（wpi）的百脉根（Lotus japonicus）MG-20成熟根瘤，开展时序转录组学研究。将供试植株置于10 mM硝酸钾（KNO3）溶液中处理6天，随后移除硝酸盐并继续培养5天。实验总体设计如下：在硝酸盐处理阶段（T0至T6）与硝酸盐移除阶段（R1至R5）每日采集根瘤样本，每个时间点均设置3次生物学重复。