Expression data for 52 microsatellite stable (MSS) primary tumors from patients with proximal colon cancer.. Homo sapiens

Samples were taken from surgically resected tumor specimens from patients with proximal colon cancer. The expression profiles were determined using the Affymetrix GeneChip Human Exon 1.0 ST Array version 2. APC gene mutation status was determined using Sanger sequencing. A classifier for APC mutation status was trained using these expression data. Overall design: 52 microsatellite stable (MSS) proximal colon cancers samples were analyzed. 17 samples were APC wild-type and 35 had APC protein-truncating mutations.

本研究样本取自近端结肠癌患者手术切除的肿瘤标本。基因表达谱通过Affymetrix GeneChip Human Exon 1.0 ST Array 版本2芯片进行检测；APC基因突变状态采用Sanger测序法确定。基于上述表达数据，训练得到可用于预测APC基因突变状态的分类器。实验整体设计：共分析52例微卫星稳定（microsatellite stable, MSS）近端结肠癌样本，其中17例为APC野生型，35例携带APC蛋白截短突变。