Expression data from CD44-knockdown MDA-MB-231 human breast cancer cells

CD44, an adhesion molecule that binds to extracellular matrix, primarily to hyaluronan (HA), has been implicated in cancer cell migration, invasion, and metastasis. CD44 has also recently been recognized as a marker for stem cells of several types of cancer. However, the roles of CD44 in the development of bone metastasis still remain unclear. To explore this issue, we established the MDA-MB-231 human breast cancer cells stably expressing short hairpin RNA against CD44. The CD44-knockdown MDA-MB-231 cells (MDA-MB-231 shCD44-2 and shCD44-3) were analyzed. As control, MDA-MB-231 cells stably expressing shRNA against firefly luciferase (shLuc) were used. Total of three samples. No replicates.

CD44（CD44）是一类可结合细胞外基质的黏附分子（adhesion molecule），其主要配体为透明质酸（hyaluronan, HA），已被证实与癌细胞的迁移、侵袭及转移过程密切相关。近年来的研究还发现，CD44可作为多种癌症干细胞的标志物。然而，CD44在骨转移（bone metastasis）发生发展中的具体作用仍不明确。为探究这一科学问题，本研究构建了稳定表达靶向CD44的短发夹RNA（short hairpin RNA, shRNA）的人乳腺癌MDA-MB-231细胞系，并对CD44敲低的MDA-MB-231细胞（MDA-MB-231 shCD44-2与shCD44-3）进行分析。实验以稳定表达靶向萤火虫荧光素酶（firefly luciferase, Luc）的shRNA的MDA-MB-231细胞（shLuc）作为对照。本研究共设置3份样本，未设置生物学重复。