Do it yourself: 3D-printed miniature CDC trap for adult mosquito (Diptera: Culicidae) surveillance

The central component of mosquito and vector surveillance programs
globally is the adult mosquito trap, which is intended to collect
host-seeking mosquitoes. The miniature CDC trap is a widely distributed
trap style in part due to its relative affordability and compact nature.
Despite already being a simple trap, in-house production methods, such as
3D printing, could improve the accessibility of the CDC trap by
eliminating some of the supply chain variables. We present here several
trials with the Salt Lake City (SLC) trap, a three-dimensional (3D)
printed trap design. Functional assessments were made on secondary
components and found no statistically significant differences when
comparing CO2 line height (above vs. below fan), battery types (sealed
lead acid vs. USB battery pack), and trap body collection shape (funnel
body vs. simple/straight body). The SLC trap was compared directly to a
commercial equivalent, the ABC trap, with comparative assessment on
species diversity and evenness in collections and found to be
statistically equivalent on all metrics. Methods also detail an
accompanying optional transport system for a pressurized CO2/regulator
set-up, should a practitioner elect not to use dry ice. Our final design
is presented here with the publicly published stereolithography (STL)
files and a detailed outline of the transport container system.
Alternative models are available for in-house manufacture of mosquito
traps, and we contribute these designs in an effort to stimulate further
growth in vector surveillance.

全球范围内蚊子及病媒监测项目的核心组成部分是成蚊诱捕器，其旨在收集寻找宿主的蚊子。微型CDC诱捕器是一种广泛使用的诱捕器类型，部分原因在于其相对经济实惠且结构紧凑。尽管该诱捕器已十分简易，但3D打印等内部生产方法可通过消除部分供应链变量，进一步提升CDC诱捕器的获取便利性。本文介绍了针对盐湖城（SLC）诱捕器的多项试验，该诱捕器为一种三维（3D）打印设计。研究对次要组件进行了功能评估，结果显示，在比较二氧化碳管路高度（风扇上方 vs 下方）、电池类型（密封铅酸电池 vs USB电池组）及诱捕器收集仓形状（漏斗形仓体 vs 简易/直筒形仓体）时，未发现统计学显著差异。研究将SLC诱捕器与商用同类产品ABC诱捕器进行直接对比，评估了两者收集样本的物种多样性及均匀度，结果显示其在所有指标上均具有统计学等效性。研究方法还详细介绍了一套配套的可选运输系统，适用于加压二氧化碳/调节器装置——若从业者选择不使用干冰时可采用该系统。本文呈现了最终设计方案，包括公开发布的立体光刻（STL）文件及运输容器系统的详细说明。目前已有适用于内部生产蚊子诱捕器的替代模型，我们贡献这些设计方案，旨在推动病媒监测领域的进一步发展。