RNA seq. Secale cereale

The economically important Ergot fungus Claviceps purpurea is an interesting biotrophic model system because of its strict organ specificity (grass ovaries) and the lack of any detectable plant defense reactions. Though several virulence factors were identified, the exact infection mechanisms are unknown, e.g. how the fungus masks its attack and if the host detects the infection at all. We present a dual transcriptome analysis using an RNAseq approach. We studied both, fungal and plant gene expression in young ovaries mock infected, infected by the wildtype and two virulence-attenuated mutants.

具有重要经济价值的麦角菌（Ergot fungus，Claviceps purpurea）是一类极具研究价值的活体营养型（biotrophic）模式研究体系，其具有严格的器官特异性，仅侵染禾本科植物的子房，且不会引发任何可检测到的植物防御反应。尽管目前已鉴定出多种毒力因子（virulence factors），但该真菌的确切侵染机制仍未阐明，例如其如何隐匿自身的侵染过程，以及宿主植物是否能够感知到侵染事件。本研究采用RNA测序（RNAseq）技术开展双转录组分析，对模拟接种组、野生型（wildtype）菌株侵染组以及两株毒力减毒突变体（virulence-attenuated mutants）侵染组的禾本科幼小子房内的真菌与植物基因表达情况进行了研究。