RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma [m6A-seq]

We here report ALKBH5, a m6A RNA demethylase, as a crucial oncogene in multiple myeloma (MM). Using various MM models, we demonstrated a critical requirement of ALKBH5 for MM cell proliferation in vitro and in vivo. To identify the potential mRNA targets of ALKBH5, we conducted m6A-seq with mRNA samples enriched from MM cells with or without ALKBH5 knockdown. m6A-seq of RPMI-8226 cells with or without shRNA-mediated ALKBH5 konckdown

本研究报道ALKBH5——一种m6A RNA去甲基化酶（m6A RNA demethylase）——为多发性骨髓瘤（multiple myeloma，MM）中的关键致癌基因。借助多种多发性骨髓瘤模型，本研究证实ALKBH5对多发性骨髓瘤细胞的体外及体内增殖均具有关键调控作用。为鉴定ALKBH5的潜在mRNA靶标，本研究针对经ALKBH5敲低与未敲低的多发性骨髓瘤细胞中富集的mRNA样本开展了m6A测序（m6A-seq）。本研究还针对经短发夹RNA（shRNA）介导敲低ALKBH5与未敲低的RPMI-8226细胞开展了m6A测序。