Raw qPCR dataset for Sirt1 expression in VPA-treated neurosurgery patients

This dataset contains the raw qPCR records for Sirt1 expression in peripheral blood of neurosurgery patients receiving valproate prophylaxis. Samples include 235 training (T001–T235) and 68 validation (V001–V068) subjects, each measured at baseline, 2 weeks, and 8 weeks. For each time point, Ct values of Sirt1 and ACTB (housekeeping control), ΔCt, ΔΔCt, and relative expression (2^-ΔΔCt) are provided. The data were generated on an Applied Biosystems 7500 Real-Time PCR System using Takara TB Green Premix Ex Taq II (Lot CN830) in 20 µL reactions, with technical triplicates and negative controls. Primer sequences and PCR efficiencies are provided in the metadata file.<br>The long-format CSV expands each sample/timepoint/target into three replicate rows; as original per-well export files are currently unavailable, replicate rows are marked with <code>Reconstructed_from_single_Ct=True</code> to indicate reconstruction from study logs. All subject IDs are de-identified.<br>This dataset supports the manuscript "Construction and Validation of an XGBoost Model for Predicting Postoperative Epileptic Seizure Risk in Neurosurgery Based on Sodium Valproate Blood Concentration and Dynamic Expression of Sirt1".

本数据集包含接受丙戊酸钠预防治疗的神经外科患者外周血中Sirt1（沉默信息调节因子1）表达的原始实时荧光定量PCR（qPCR）记录。样本分为235份训练集（编号T001–T235）与68份验证集（编号V001–V068），所有样本均在基线期、术后2周及术后8周三个时间点完成检测。针对每个时间点，本数据集均提供Sirt1与持家基因对照ACTB的Ct值、ΔCt值、ΔΔCt值以及相对表达量（2^-ΔΔCt）。本实验采用Applied Biosystems 7500型实时荧光定量PCR系统，使用Takara TB Green Premix Ex Taq II（批号CN830）配置20μL反应体系，设置技术重复三次与阴性对照，引物序列及PCR扩增效率详见元数据文件。
本数据集采用长格式CSV文件存储，将每个样本/时间点/靶标扩展为三条重复行；由于原始单孔导出文件目前无法获取，重复行均标注有`Reconstructed_from_single_Ct=True`以表明其由研究日志重构而来。所有受试者ID均已完成去标识化处理。
本数据集可支撑论文《基于丙戊酸钠血药浓度与Sirt1动态表达构建并验证XGBoost模型预测神经外科术后癫痫发作风险》的相关研究工作。