Real-time quantitative PCR analysis of miRNAs in HIV patients who did (cases) or did not (controls) experience Type 1 MI. Real-time quantitative PCR analysis of miRNAs in HIV patients who did (cases) or did not (controls) experience Type 1 MI

RT-PCR of platelet-poor plasma samples per Freedman JE, Gerstein M, Mick E, Rozowsky J, Levy D, Kitchen R, et al. Diverse human extracellular RNAs are widely detected in human plasma. Nat Commun 2016; 7. doi:10.1038/ncomms11106 Overall design: miRNA expression profiling from platelet poor plasma samples

本实验参照Freedman JE、Gerstein M、Mick E、Rozowsky J、Levy D、Kitchen R等建立的方法，对乏血小板血浆（platelet-poor plasma）样本进行逆转录聚合酶链式反应（Reverse Transcription Polymerase Chain Reaction, RT-PCR）检测。相关参考文献：Freedman JE 等. 人类血浆中广泛检测到多样化的人类细胞外RNA（Diverse human extracellular RNAs are widely detected in human plasma）. 自然-通讯（*Nature Communications*）, 2016年，第7卷. DOI: 10.1038/ncomms11106。实验整体设计：基于乏血小板血浆样本的微小RNA（microRNA, miRNA）表达谱分析。