Crosslinking Mass Spectrometry

Crosslinking mass spectrometry collected on Lumos Orbitrap elite with HCD and UVPD. BS3 and DMTMM were used for crosslinking separately.

本数据集采用搭载高能碰撞解离（High-energy Collisional Dissociation，HCD）与紫外光解离（Ultraviolet Photodissociation，UVPD）模块的Lumos Orbitrap Elite质谱仪完成交联质谱（Crosslinking Mass Spectrometry）数据采集；实验中分别以双(磺基琥珀酰亚胺基)辛二酸酯（Bis(sulfosuccinimidyl) suberate，BS3）和4-(4,6-二甲氧基三嗪-2-基)-4-甲基吗啉鎓氯化物（4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride，DMTMM）作为交联试剂开展交联反应。