RNA sequencing analysis of A549 cells under glutamine deprivation

We performed RNA-seq ananlysis to idendity lncRNAs regulated by glutamine deprivation in A549 cells. Overall design: RNA-seq was performed on A549 cells cultured in complete medium(ctrl) or glutamine-depleted medium(QD) for 24 hours, and then we compared gene expression profiling analysis of ctrl and QD RNA-seq data.

本研究开展RNA测序（RNA-seq）分析，旨在鉴定A549细胞中受谷氨酰胺剥夺调控的长链非编码RNA（lncRNAs）。实验整体设计：将A549细胞分别培养于完全培养基（对照组，ctrl）与谷氨酰胺剥夺培养基（QD）中培养24小时，随后对两组样本的RNA-seq数据开展基因表达谱对比分析。