Raw images of immunoprecipitation experiments for Figure 3, immunoprecipitation

Uncropped data from Figure 3 (A) and replicate (C) for VAMP7 immunoprecipitation from Cos-7 cell lysate overexpressing GFP-tagged mouse, rat or human VAMP7 constructs. Uncropped immunoblotting data from Figure 3 (B) and replicate (D) for VAMP7 immunoprecipitation from WT and VAMP7 KO mouse cortex extracts. Antibodies used for immunoprecipitation and subsequent immunoblotting are indicated. Red dashed lines show GFP-VAMP7 protein and cropped region, respectively. IN=Input (50 µg in A and C, 100 µg in B and D); SN = supernatant after immunoprecipitation; IP = immunoprecipitate; * = GFP-VAMP7; ° = Absence of band at GFP-VAMP7 size (~50 kDa); ~: immunoglobulins.

针对过表达绿色荧光蛋白（Green Fluorescent Protein, GFP）标签小鼠、大鼠或人源VAMP7重组载体的Cos-7细胞裂解液开展的VAMP7免疫沉淀（immunoprecipitation）实验，其图3(A)及重复实验(C)的未裁切原始数据。针对野生型（wild type, WT）与VAMP7基因敲除（knockout, KO）小鼠皮层提取物开展的VAMP7免疫沉淀实验，其图3(B)及重复实验(D)的未裁切免疫印迹（immunoblotting）原始数据。免疫沉淀及后续免疫印迹所用的抗体已标注。红色虚线分别指示GFP-VAMP7蛋白与裁切区域。IN=上样对照（A、C组为50 μg，B、D组为100 μg）；SN=免疫沉淀后的上清液；IP=免疫沉淀复合物；*代表GFP-VAMP7；°代表GFP-VAMP7分子量约50 kDa处无条带；~代表免疫球蛋白。