Hepatic gene expression in male fetuses from maternal nutrient restriction in mice. Hepatic gene expression in male fetuses from maternal nutrient restriction in mice

The aim of this study was to identify fetal adaptations to gene expression in our mouse model of intrauterine growth restriction (IUGR). Starting at E6.5, pregnant CD-1 mice received either control (ad libitum) or 70% of ad libitum food (maternal nutrient restriction [MNR]). At E18.5, fetal right liver lobes were collected from 2 pups/litter in litters with 11-15 pups. Frozen liver was homogenized for RNA isolations. Isolated RNA was sequenced on a HiSeq2500 with an average read depth of 23M reads and a range of 17-31M reads per sample. Reads were aligned to the mm9 transcriptome with Bowtie2. Normalization and differential expression was done with DESeq2, ALDex2 and EdgeR, and genes were considered in the study if they were consistent in 2 or more tools (FDR <0.1). Overall design: 10 control and 10 MNR samples were analyzed with (2 pups/ litter)

本研究旨在探究宫内生长受限（intrauterine growth restriction, IUGR）小鼠模型中胎儿的基因表达适应性改变。自胚胎第6.5天（E6.5）起，将受孕的CD-1小鼠随机分为两组：对照组给予自由进食（ad libitum）饲料，处理组给予相当于自由进食量70%的饲料（即母体营养限制（maternal nutrient restriction, MNR））。于胚胎第18.5天（E18.5），从每窝产仔数为11~15只的幼崽中，每窝采集2只幼崽的右侧肝叶。将冻存的肝脏组织匀浆后提取总RNA，提取的总RNA在HiSeq2500测序平台上进行测序，所有样本的平均测序深度为23M reads，单样本reads数范围为17~31M。使用Bowtie2将测序reads比对至mm9转录组，随后采用DESeq2、ALDex2及EdgeR进行数据标准化与差异表达分析。若基因在至少2种分析工具中结果一致且错误发现率（false discovery rate, FDR）<0.1，则将其纳入本研究。实验整体设计如下：共分析10个对照组样本与10个MNR组样本，每组样本均来自每窝2只幼崽的肝组织。