Fibrous PCL/PLLA Scaffolds Obtained by Rotary Jet Spinning and Electrospinning

Rotary jet spinning (RJS) and electrospinning are techniques to obtain fibrous scaffolds. RJS is a simple method, which fabricates three-dimensional fibers by exploiting a high-speed rotating nozzle, creating a polymer jet which stretches until solidification, and does not require high voltage. In opposite, electrospinning technique needs the presence of an external electric field to create fiber from the polymeric jet solution. This article investigates both processes using two different biocompatible polymers: Poly(L-lactic acid) (PLLA) and Poly(ε-caprolactone) (PCL). Samples were characterized by scanning electron microscopy, thermogravimetric analysis, differential scanning calorimeter, and Fourier-transform infrared spectroscopy. Morphological observations showed the efficiency of both techniques in obtaining nanofibers. Thermal analyses of data indicate immiscible property of different blends and the total solvent evaporation. In vitro cytocompatibility test showed that RJS and electrospinning samples exhibited good cytocompatibility. Based on these results, it may be concluded that the fibers obtained with both technologies are non-cytotoxicity and with good biocompatibility, and might be suitable for applications as scaffold for cell growth.

旋转喷射纺丝（Rotary Jet Spinning, RJS）与静电纺丝均为制备纤维支架的技术手段。旋转喷射纺丝是一种简便工艺，借助高速旋转喷嘴制备三维纤维：通过形成聚合物射流并使其拉伸直至固化，且无需施加高压电场。与之相对，静电纺丝技术则需要借助外部电场，从聚合物射流溶液中制备纤维。本研究采用两种不同的生物相容性聚合物——聚L-乳酸（Poly(L-lactic acid), PLLA）与聚ε-己内酯（Poly(ε-caprolactone), PCL），对这两种纺丝工艺展开探究。实验样品通过扫描电子显微镜（scanning electron microscopy）、热重分析（thermogravimetric analysis）、差示扫描量热法（differential scanning calorimeter）以及傅里叶变换红外光谱（Fourier-transform infrared spectroscopy）进行表征。形貌观测结果表明，两种工艺均可高效制备纳米纤维。热分析数据显示，不同共混物呈现不相容特性，且溶剂已完全挥发。体外细胞相容性测试结果表明，旋转喷射纺丝与静电纺丝制备的样品均具备良好的细胞相容性。综合上述结果可得出结论：两种工艺制备的纤维均无细胞毒性且生物相容性优异，有望用作细胞生长支架的候选应用材料。