Genes differentially expressed Haloferax mediterranei strains: control wild-type strain vs ΔphaEC strain

Transcriptional profiling of Haloferax mediterranei comparing control wild-type strain with ΔphaEC strain, in which PHA synthase genes are knockouted. ΔphaEC strain is deficient in PHBV accumulation. Goal was to explore the PHBV biosynthetic pathway and to determine their impact on primary metabolism in H. mediterranei. Total RNA from the control Haloferax mediterranei and its ΔphaEC strain were used to generate target cDNA, and then hybridized to 8*15K Haloferax mediterranei genome array genechips, representing about 3800 genes.

本数据集为地中海嗜盐古菌（Haloferax mediterranei）的转录谱分析，对比野生型对照菌株与ΔphaEC敲除菌株：该敲除菌株的PHA合酶基因已被敲除，无法积累聚羟基丁酸戊酸共聚酯（PHBV）。本研究旨在探索PHBV的生物合成途径，并明确其对地中海嗜盐古菌初级代谢的影响。实验中，提取野生型对照菌株与ΔphaEC菌株的总RNA以制备目标cDNA，随后将其与8×15K地中海嗜盐古菌基因组基因芯片进行杂交，该芯片覆盖约3800个基因。