The Schizophrenia Risk Gene Product Satb2 Regulates miRNAs Expression and Long-Term Memory in Adult CNS

(i) The hippocampal transcriptome was analyzed in mice that lack the SatB2 gene (ii) Satb2 binds to active promoters of protein-coding and non-coding loci and determines the levels of a large cohort of miRNAs in the CA1 hippocampal field that are implicated in synaptic plasticity and/or memory formation. (i) RNA was isolated from the hippocampal CA1 region of mice that lack the SatB2 gene from the adult forebrain and subjected to RNA-sequencing (ii) Mapping the genomic localization of Satb2 in mouse primary hippocampal neurons.

(i) 针对SatB2基因缺失的小鼠，对其海马转录组进行分析；
(ii) SatB2可结合编码蛋白基因座与非编码基因座的活性启动子，并调控海马CA1区大量参与突触可塑性和/或记忆形成的微小RNA（miRNA）的表达水平；
(i) 从成年前脑SatB2基因缺失小鼠的海马CA1区分离总RNA，并开展RNA测序（RNA-sequencing）；
(ii) 绘制小鼠原代海马神经元中SatB2的基因组定位图谱