Injured sensory neurons-derived Galectin-3 contributes to neuropathic pain via programming microglia in the spinal dorsal horn

Gal3 plays an important role in chronic pain through the activation of microglia. We used single nucleus RNA sequencing (snRNA-Seq) to uncover the mechanisms of Gal3-activated microglia in pain regulation. Spinal dorsal horn (SDH) from 6-days vehicle or Gal3-treated mice, 3 male mice for each group were used to perform the snRNA-Seq (2 groups: vehicle control (1 sample containing with 3 male mice's SDH) and Gal3-treatment (1 sample containing 3 male mice's SDH)).

半乳糖凝集素3（Gal3）通过激活小胶质细胞在慢性疼痛中发挥重要作用。本研究采用单细胞核RNA测序（single nucleus RNA sequencing, snRNA-Seq），旨在阐明Gal3激活的小胶质细胞参与疼痛调控的分子机制。我们采集了6日龄经赋形剂或Gal3处理的小鼠脊髓背角（spinal dorsal horn, SDH）组织，每组选取3只雄性小鼠，最终构建两组snRNA-Seq样本：赋形剂对照组（1份样本包含3只雄性小鼠的脊髓背角组织）与Gal3处理组（1份样本包含3只雄性小鼠的脊髓背角组织）。