scRNAseq of Primary and Immortalized Human Prostate Fibroblast Cell Lines

Single cell gene expression data for multiple human prostate fibroblast cell lines including primary human prostate fibroblasts from a young adult male before and after immortalization via EF1a-driven hTERT lentiviral construct (pHPF and iHPF), HPV E6/E7-immortalized prostate stromal fibroblasts from a stromal nodule of benign prostatic hyperplasia (N1), and spontaneously immortalized prostate fibroblasts from a solitary fibrous tumor (SFT-1). Sequencing data was used to characterize transcriptomes and to infer transcription factor activity using a novel noisy Boolean logic Bayesian model to investigate fibroblast phenotypic plasticity.

本数据集包含多株人前列腺成纤维细胞系的单细胞基因表达数据（single cell gene expression data），具体涵盖三类样本：其一为来自年轻成年男性的原代人前列腺成纤维细胞，经EF1α驱动的hTERT慢病毒载体（lentiviral construct）永生化前后的样本，分别标记为pHPF与iHPF；其二为源自良性前列腺增生（benign prostatic hyperplasia）间质结节的HPV E6/E7永生化前列腺间质成纤维细胞，标记为N1；其三为来自孤立性纤维瘤（solitary fibrous tumor）的自发永生化前列腺成纤维细胞，标记为SFT-1。研究人员利用配套的测序数据对转录组进行表征，并通过一种新型噪声布尔逻辑贝叶斯模型（noisy Boolean logic Bayesian model）推断转录因子（transcription factor）活性，以探究成纤维细胞的表型可塑性（phenotypic plasticity）。