Biosurveillance for invasive insect pest species using an environmental DNA metabarcoding approach and a high salt trap collection fluid

Amplification of environmental DNA from a saturated salt solution, trap fluid allows for the retention of trap specimens for downstream morphological identifications. In addition, a saturated salt solution to preserve specimens in traps addresses issues with the high evaporation rate of ethanol, and public safety concerns with other fluid preservation options in public settings. The current repository contains metabarcoding and associated data for a 407-nucleotide segment of the cytochrome c oxidase subunit 1 (COI) animal barcode region. Fluid was collected from 12 Lindgren funnel traps in two Ontario regions in forested areas to survey for wood boring beetles of regulatory concern. Our results show how this protocol can be used as an efficient method for the surveillance of insects using a trap with a saturated salt solution and eDNA metabarcoding to detect species of regulatory concern.

本研究针对饱和盐溶液中的环境DNA（environmental DNA, eDNA）进行扩增，采集的诱捕液可留存诱捕所得标本，用于后续形态学鉴定。此外，采用饱和盐溶液保存诱捕器内标本的方案，可解决乙醇蒸发速率过快的问题，同时规避了其他液体保存方式在公共场景下存在的公共安全隐患。本数据集包含细胞色素c氧化酶亚基1（cytochrome c oxidase subunit 1, COI）动物条形码区域一段407个核苷酸片段的宏条形码（metabarcoding）测序数据及配套关联数据。研究人员从加拿大安大略省两个林区的12个林德格伦漏斗诱捕器（Lindgren funnel trap）中采集诱捕液，针对具有监管关注度的蛀木甲虫类群开展调查。研究结果表明，该方案可作为一种高效的昆虫监测手段：通过配备饱和盐溶液的诱捕器结合环境DNA宏条形码技术，可检测具有监管关注度的昆虫物种。