Additional file 3: of Development of EMS-induced mutation population for amylose and resistant starch variation in bread wheat (Triticum aestivum) and identification of candidate genes responsible for amylose variation

Normalized threshold cycle (ΔCт) of 20 starch metabolic genes during seed development. The data was taken at three stages of seed development (21, 28, and 35 days after anthesis, DAA) in two mutant lines, ‘TAC 75’(amylose content - 64 %) and ‘TAC 6’(amylose content– 6.8 %), in comparison to the parent variety, ‘C 306’ (amylose content - 26 %). The data was taken in two biological replications, each represented as mean ± SD from three technical replicates. Wheat ADP Ribosylation Factor (ARF) was used as an internal control gene for normalization. (XLSX 23 kb)

本数据集涵盖20个淀粉代谢基因在种子发育过程中的归一化循环阈值（ΔCt）值。实验以两个突变株系‘TAC 75’（直链淀粉含量64%）、‘TAC 6’（直链淀粉含量6.8%）及其亲本品种‘C 306’（直链淀粉含量26%）为材料，采集了三者在三个种子发育阶段的样本，三个阶段分别为开花后21、28、35天（DAA）。所有数据均设置两次生物学重复，每个重复的结果以三次技术重复的平均值±标准差（SD）表示。实验以小麦ADP核糖基化因子（ADP Ribosylation Factor，ARF）作为归一化内参基因。（XLSX格式，大小23 KB）