Atrx deletion impairs cGAS-STING signaling and increases response to radiation and oncolytic herpesvirus in soft tissue sarcoma. Atrx deletion impairs cGAS-STING signaling and increases response to radiation and oncolytic herpesvirus in soft tissue sarcoma

RNA sequencing of isogenic ATRX wild-type (WT) and ATRX knockout (KO) paired isogenic cell lines treated with either 1.)interferon stimulatory DNA (ISD) and harvested 24 hours after treatment, 2)4 Gy ionizing radiation and harvested 36 hours after treatment, or 3) untreated control. These experiments help determine the differential impact of ATRX mutational status on cGAS-STING and type-I interferon signaling in soft tissue sarcoma. Overall design: RNA sequencing of ATRX WT and ATRX KO soft tissue sarcoma cell lines

本数据集针对配对同基因ATRX野生型（WT）与ATRX敲除（KO）软组织肉瘤细胞系开展RNA测序，所有细胞系分别接受以下三种处理：1. 干扰素刺激DNA（interferon stimulatory DNA，ISD）处理，于处理后24小时收获细胞；2. 4 Gy电离辐射处理，于处理后36小时收获细胞；3. 未处理的空白对照组。本实验旨在明确ATRX突变状态对软组织肉瘤中cGAS-STING通路与I型干扰素（type-I interferon）信号通路的差异化影响。整体实验设计：对ATRX WT与ATRX KO的软组织肉瘤细胞系进行RNA测序。