Examination of protein expression patterns of closely related Salmonella serovars using 2D GE and MS/MS. Examination of protein expression patterns of closely related Salmonella serovars using 2D GE and MS/MS

The objective of the present investigation was to consider the level of variation in the protein expression patterns of closely related Salmonella serovars, in order to search for protein factors with levels of expression or posttranslational modifications characteristic for each serovar. For the comparative expression analysis we have utilised classic 2D GE approach which revealed several proteins with serovar specific expression as well as proteins which do not alter their expression levels between serovars and strains. The proteins of interest were identified using LC/MS/MS. Keywords: 2D GE, MS/MS Overall design: Analysis of 12 strains of S. enterica representing five different serovars.

本研究旨在探究亲缘关系相近的沙门氏菌血清型的蛋白质表达谱差异水平，以期筛选出各血清型特有的表达水平或翻译后修饰特征的蛋白质因子。
本研究采用经典的二维凝胶电泳（2D GE）方法开展比较表达分析，不仅鉴定出了若干具有血清型特异性表达的蛋白质，同时也发现了在不同血清型与菌株间表达水平无显著变化的蛋白质。
本研究采用液相色谱-串联质谱（LC/MS/MS）技术对目标蛋白质进行了鉴定。
关键词：二维凝胶电泳（2D GE）、串联质谱（MS/MS）
实验整体设计：对代表5种不同血清型的12株肠炎沙门氏菌（S. enterica）进行分析。