Expression data from effects of Ganoderma lucidum polysaccharides F3 on human monocytic leukemia cell line THP-1. Homo sapiens

In order to identify patterns of gene expression associated with biological effects in THP-1 cells induced by F3, we performed a transcriptomic analysis on the THP-1 control and F3-treated THP-1 cells by oligonucleotide microarray Overall design: 10^7 cells/mL concentrations of THP-1 cells were seeded in 100 mm dish and incubated overnight. After that, cells were treated with F3 at a final concentration of 30 ug/mL. After incubated for 6 and 24 hours, the cell pellets were collected by centrifugation at 250g for 5 min, correspondingly. Controlled samples of un-induced cells were treated in the same way with the same amount of medium.

为鉴定F3诱导THP-1细胞产生生物学效应相关的基因表达模式，我们通过寡核苷酸微阵列（oligonucleotide microarray）对对照组THP-1细胞与F3处理组THP-1细胞开展转录组学分析。实验设计如下：将浓度为10^7个细胞/mL的THP-1细胞接种于100 mm培养皿中，过夜培养。随后以终浓度30 μg/mL的F3处理细胞，分别于处理6小时和24小时后，以250g离心5分钟收集细胞沉淀。未诱导的对照组细胞则采用等量培养基进行同等条件处理。