Multi-omics analysis revealed the potential role of rumen microbes in heat stress

Heat stress has been an important issue in dairy cattle feeding management affecting summer health and economic efficiency. In recent years, global climate change has led to an increase in atmospheric CO2 and average daily temperature, making heat stress a major challenge in dairy farming. In this experiment, 10 cows each of growing heifers, heifers, and lactating cows were selected for sample collection in April and August , respectively. Rumen fluid were collected, and gauze filtered to obtain rumen fluid immediately transferred to a liquid nitrogen tank for measurement of 16SrDNA. Here, we characterized both the changes in rumen microbial communities potential derived from rumen fluid samples in cows during different growth stages and climates.

热应激始终是奶牛饲养管理领域的关键议题，其对夏季奶牛健康与养殖经济效益均存在显著负面影响。近年来，全球气候变化导致大气二氧化碳浓度与日均气温持续攀升，使得热应激成为奶牛养殖业面临的重大挑战。本实验分别选取育成青年母牛、青年母牛及泌乳母牛各10头，于4月与8月开展样本采集工作。采集瘤胃液后经纱布过滤获得瘤胃液样本，立即将其转移至液氮罐中保存，以待开展16S核糖体DNA（16SrDNA）检测分析。本研究针对不同生长阶段与气候环境下的奶牛瘤胃液样本，解析了其瘤胃微生物群落的潜在变化特征。