CIL:6195, Rattus, multipolar neuron. In Cell Image Library

Synapse formation in cultured hippocampal neurons after 7 days in vitro. Cells were immunostained for MAP2, a microtubule associated protein localized to dendrites but not axons (green), and synapsin I, a presynaptic vesicle protein (red)to reveal the development of presynaptic contacts along the dendritic arbor.该数据集展示体外培养7天的海马神经元的突触形成情况。实验中对细胞进行免疫荧光染色：以绿色荧光标记微管相关蛋白2（MAP2，microtubule-associated protein），该蛋白特异性定位于树突而非轴突；以红色荧光标记突触前囊泡蛋白突触蛋白I（synapsin I），从而揭示树突丛上突触前接触位点的发育进程。