Changes in protein expression in hrpA mutant clones relative to wild-type B. burgdorferi as estimated by 2D gel electrophoresis.

Changes in protein expression in hrpA mutants relative to wild-type B. burgdorferi as determined by 2D gel electrophoresis. Average fold increase or decrease was estimated as the mean value from two gels of the ratio between the spot volumes in wild-type Borrelia burgdorferi B31 and the hrpA mutant strains hrpA2 (GCB1164), hrpA3 (GCB1165) and hrpA4 (GCB1166). Spot volumes were estimated using REDFIN 2D gel analysis software.*indicates the proteins identified with both 2D gel and iTRAQ analysis.NA indicates spots that were not identified. The 20 most abundant spots with a changed expression pattern in the mutants were identified by LC MS/MS analysis as described in materials and methods.

本数据集通过双向凝胶电泳（2D gel electrophoresis）分析了hrpA突变株相较于野生型伯氏疏螺旋体（Borrelia burgdorferi）的蛋白质表达差异。蛋白的平均上调或下调倍数通过野生型伯氏疏螺旋体B31与hrpA突变株hrpA2（GCB1164）、hrpA3（GCB1165）及hrpA4（GCB1166）的斑点体积比值的均值估算得到，每组实验重复两次凝胶电泳。斑点体积采用REDFIN二维凝胶分析软件进行定量。* 表示通过双向凝胶电泳与同重同位素相对和绝对定量（iTRAQ）联合鉴定的蛋白质。NA表示未完成鉴定的蛋白斑点。本研究参照材料与方法所述流程，通过液相色谱-串联质谱（LC-MS/MS）分析鉴定了突变株中表达模式发生改变且丰度最高的20个蛋白斑点。