Resequencing of Turicibacter sanguinis MOL361

Nanopore long read sequencing was conducted to complete the genome of Turicibacter sanguinis MOL361. These reads were assembly in unicycler with the original HiSeq reads from bioproject PRJNA500327 to produce a complete genome with two contigs. However, quality control revealed the small second plasmid to be a contaminant. This left one closed, circular chromosome for T. sanguinis MOL361

本研究采用纳米孔长读长测序（Nanopore long read sequencing）技术完成了血Turicibacter MOL361（Turicibacter sanguinis MOL361）的基因组组装工作。将该纳米孔测序所得读段与来自生物项目PRJNA500327的原始HiSeq测序读段（HiSeq reads）一同使用Unicycler软件进行组装，得到包含两个重叠群（contig）的完整基因组。然而，经质量控制（quality control）分析显示，其中较小的第二条质粒（plasmid）为污染物。最终，血Turicibacter MOL361仅保留一条闭合环状染色体（closed circular chromosome）。