Metagenomic sequencing of cigarette smoke-exposed mouse gut microbiome. mouse gut metagenome

To determine the role of the microbiome in the development of COPD, laboratory mice were exposed either to cigarette smoke or to faecal pellets from cigarette smoke exposed mice. In parallel, cigarette smoke exposed mice were treated with faecal pellets from non-smoke-exposed mice to determine whether any restorative effect was evident. Faecal samples were collected across 8 or 12 weeks.

为明确微生物组（microbiome）在慢性阻塞性肺疾病（COPD）发生发展中的作用，本研究将实验小鼠分为两组，一组暴露于香烟烟雾，另一组接受香烟烟雾暴露小鼠的粪便颗粒移植。与此同时，对香烟烟雾暴露小鼠施以非烟雾暴露小鼠的粪便颗粒干预，以验证是否存在修复效应。实验期间，分别在第8周和第12周采集粪便样本。