miRNA Expression from Cerebrospinal Fluid (CSF) from Prodromal Huntington Disease Patients. miRNA Expression from Cerebrospinal Fluid (CSF) from Prodromal Huntington Disease Patients

Analysis of microRNA (miRNA) expression in CSF from prodromal Huntington disease patients. Overall design: miRNA levels were measured in CSF from 60 PREDICT-HD study participants using the HTG protocol. Using a CAG–Age Product score, 30 prodromal HD participants were selected based on estimated probability of imminent clinical diagnosis of HD (i.e., low, medium, high; n = 10/group). For comparison, participants already diagnosed (n = 15) and healthy controls (n = 15) were also selected.

本研究对前驱期亨廷顿舞蹈病（Huntington disease，HD）患者脑脊液（cerebrospinal fluid，CSF）中的微小RNA（microRNA，miRNA）表达水平进行分析。实验设计概述：采用HTG实验方案，对60名参与PREDICT-HD研究的受试者的脑脊液样本中的miRNA水平开展检测。基于CAG-年龄乘积评分，根据受试者即将发生HD临床确诊的预估概率，本研究选取30名前驱期HD受试者，分为低、中、高风险三组，每组各10例。为设置对照，同时纳入15名已确诊HD的受试者以及15名健康对照者。