Gene activity induced by denervation in the rat urinary bladder [mRNA]

Bilateral freezing of the pelvic ganglia in female rats were performed to denervate the urinary bladder. Sham operated rats were used as controls. The rats were sacrificed 10 days after surgery. The urinary bladders (including the urothelium) were frozen and used for RNA extraction.本研究通过对雌性大鼠双侧盆腔神经节进行冷冻处理，以实现膀胱去神经支配。以假手术大鼠作为对照组。术后10天处死所有大鼠，摘取包含尿路上皮（urothelium）的膀胱组织并冷冻保存，用于RNA提取。