IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase

Abstract INTRODUCTION: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. METHODS: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity. RESULTS: Most of the schistosomiasis patient’s (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production. CONCLUSIONS: Potato apyrase and SmATPDases have IgE-binding epitopes.

摘要 引言：已有研究表明，马铃薯腺苷三磷酸二磷酸水解酶（potato apyrase）与曼氏血吸虫（Schistosoma mansoni）ATP二磷酸水解酶（SmATPDases）同工型之间存在高比例的结构同源性与交叉免疫反应性，提示两类蛋白共享特定表位。
方法：采用酶联免疫吸附试验（ELISA）、蛋白质免疫印迹（western blot）及小鼠免疫实验方法，以马铃薯腺苷三磷酸二磷酸水解酶为研究对象验证其IgE反应性。
结果：75%的血吸虫病患者血清对马铃薯腺苷三磷酸二磷酸水解酶呈血清学阳性，且该蛋白可通过蛋白质免疫印迹法被识别，提示寄生虫与植物蛋白共享IgE结合表位。
结论：马铃薯腺苷三磷酸二磷酸水解酶与SmATPDases均含有IgE结合表位。