mRNA translation efficiency analysis in liver at 0 h and 24 h after 70% partial hepatectomy

In order to detect changes in liver tissue protein translation efficiency during liver regeneration, we performed sucrose density gradient centrifugation to separate translated (associated with polysome fraction), and non-translated (associated with sub-polysome fraction) transcripts in extracts from mouse livers 0 h and 24 h post-surgery. We found that compared to liver tissues before surgery, extracts from tissues 24 h post-surgery had significantly fewer transcripts associated with polysomes, suggesting a reduction in protein synthesis activity. Overall design: Liver polysome and sub-polysome RNA profiles of mice at 0 h and 24 h after partial hepatectomy were generated by deep sequencing. Three samples from each group were included for sequencing.

为探究肝再生过程中肝组织蛋白质翻译效率的变化，我们采用蔗糖密度梯度离心法，从术后0小时与24小时的小鼠肝脏提取物中，分离出与多聚核糖体（polysome）组分结合的翻译中转录本，以及与亚多聚核糖体（sub-polysome）组分结合的非翻译转录本。结果显示，与手术前的肝组织相比，术后24小时的组织提取物中与多聚核糖体结合的转录本数量显著减少，提示蛋白质合成活性有所降低。总体实验设计：通过深度测序构建了部分肝切除术（partial hepatectomy）后0小时与24小时的小鼠肝脏多聚核糖体与亚多聚核糖体RNA图谱。每组设置3个样本用于测序。