BATSeq of ESC-FCS/ESC-2i/NSC. Mus musculus

Single-cell polyadenylation site quantification Overall design: 48 single cells each of murine embryonic stem cells maintained in FCS+LIF medium ("ESC"), embryonic stem cells maintained in serum free, 2i containing medium ("2i") and neural stem cells ("NSC") were sequenced by BATSeq, a single cell transcriptomic protocol for mapping polyadenylation sites in single cells.

单细胞聚腺苷酸化位点定量
整体实验设计：分别对48个维持于胎牛血清（Fetal Calf Serum, FCS）联合白血病抑制因子（Leukemia Inhibitory Factor, LIF）培养基中的小鼠胚胎干细胞（Embryonic Stem Cell, ESC）、48个培养于无血清且添加2i培养基的小鼠胚胎干细胞（2i组），以及48个神经干细胞（Neural Stem Cell, NSC），采用BATSeq技术进行测序。BATSeq是一种用于在单细胞中绘制聚腺苷酸化位点的单细胞转录组学实验方案。