Energy starvation in Daphnia magna from exposure to a lithium cobalt oxide nanomaterial. Energy starvation in Daphnia magna from exposure to a lithium cobalt oxide nanomaterial

We determined lithium cobalt oxide LCO’s effects on pathways in the model organism Daphnia magna through RNA-Seq global gene expression analysis. Overall design: Examination of LCO exposure effect on gene transcription after 48 hr exposure. 5 replicates each of: no treatement control, LCO 1 mg/L, LCO 10 mg/L, and ion control equivelent to Li and Co released from 10 mg/L LCO at 48 hrs.

本研究通过全局基因表达RNA测序（RNA-Seq）分析，明确了锂钴氧化物（LCO）对模式生物大型溞（Daphnia magna）体内通路的影响。实验设计概况：考察48小时LCO暴露对基因转录的影响。实验共设置四组，每组均设置5次生物学重复：无处理空白对照组、1 mg/L LCO暴露组、10 mg/L LCO暴露组，以及离子对照组——该对照组的锂、钴离子浓度与48小时时从10 mg/L LCO中释放的锂、钴离子浓度相当。