Deep sequencing of the small RNA transcriptome of blood leukocytes from Parkinson's disease patients prior to and following deep brain stimulation (DBS) and as compared with healthy control volunteers. Homo sapiens

We report the application of small RNA sequencing technology for high-throughput profiling of microRNA molecules (miRNAs) in blood leukocytes of Parkinson's Disease (PD) patients. Expression changes which may contribute to PD pathogenesis and reaction to treatment were identified in RNA preparations from blood leukocyte samples collected from PD patients prior to and following DBS neurosurgery on stimulation and following a short one hour cessation of the electrical stimulation (which enabled dissociation of the surgery effects from the electrical stimulation alone) and from matched healthy control volunteers, all under signed informed consents. Overall design: Examination of four states: Parkinson's Disease (PD) patients 1 day prior to DBS, PD patients a few weeks after DBS neurosurgery (upon symptoms stabilization) both on and following one hour electrical stimulation cessation, and age-matched healthy control volunteers.

本研究报道了小RNA测序（small RNA sequencing）技术在帕金森病（Parkinson's Disease, PD）患者血液白细胞内微小RNA（microRNA, miRNAs）高通量表达谱分析中的应用。本研究从签署书面知情同意书的帕金森病患者及匹配的健康对照志愿者的血液白细胞RNA提取物中，鉴定出了可能参与PD发病机制及与治疗反应相关的表达差异。其中帕金森病患者的样本采集节点包括：深部脑刺激（Deep Brain Stimulation, DBS）神经外科手术电刺激前、术后，以及短暂停止电刺激1小时后——该采样设计可区分手术本身与单纯电刺激所产生的效应。实验整体设计：共涵盖4组样本状态：① DBS手术前1天的帕金森病患者；② DBS神经外科术后数周、症状已稳定且处于电刺激状态的帕金森病患者；③ DBS神经外科术后数周、症状已稳定且停止电刺激1小时后的帕金森病患者；④ 年龄匹配的健康对照志愿者。