Expression data from human esophageal cancer cells

When esophageal cancer cells are exposed to a certain dose of radiation, a series of biological process changes, including DNA damage, DNA repair, apoptosis, cell cycle, repopulation, mitotic disasters, etc. These changes may determine the radiotherapy sensitivity to esophageal cancer, as well as recurrence and metastasis after radiotherapy. To clarify the changes in gene expression in esophageal cancer after radiotherapy, we used gene expression microarrays to screen for significant up-regulation and down-regulation of genes and to analyze associations with these biological processes. Test groups(T) and Control groups(C) were selected for RNA extraction and hybridization on Affymetrix Clariom S Assay. Test groups(T): 2 flasks of cells were given a radiation dose of 18 Gy at 6 frequencies. Control groups(C): 2 flasks of cells were cultured normally.

当食管癌细胞暴露于特定剂量辐射时，会触发一系列生物学过程改变，包括DNA损伤（DNA damage）、DNA修复（DNA repair）、细胞凋亡（apoptosis）、细胞周期（cell cycle）、细胞再增殖（repopulation）以及有丝分裂灾难（mitotic disasters）等。此类改变可决定食管癌的放疗敏感性，同时影响放疗后的复发与转移情况。为明确放疗后食管癌细胞的基因表达变化规律，本研究借助基因表达微阵列（gene expression microarrays）技术筛选显著上调及下调的差异基因，并分析其与上述生物学过程的关联。实验设置实验组（T组）与对照组（C组），提取RNA后在Affymetrix Clariom S Assay平台上进行杂交反应。实验组（T组）：2瓶细胞以6个分次剂量接受18 Gy辐射照射。对照组（C组）：2瓶细胞进行常规体外培养。