Glucose partitioning in the bone marrow micro-environment in acute myeloid leukaemia [single-cell RNA-seq]

To identify the metabolic transcriptome landscape within leukaemic cells we performed scRNA-Seq on the sorted leukaemia cells (GFP+) cells from MLL-AF9 mice. MLL-AF9/GFP driven leukaemic cells were isolated by FACS-sorted GFP+ cells and analyzed using scRNAseq.

为阐明白血病细胞内的代谢转录组图谱，我们对来自MLL-AF9小鼠的经分选获得的绿色荧光蛋白（Green Fluorescent Protein，GFP）阳性白血病细胞进行了单细胞RNA测序（single-cell RNA sequencing，scRNA-Seq）。具体而言，我们通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS）分离得到由MLL-AF9/GFP驱动的白血病细胞中的GFP+亚群，并利用scRNA-Seq对其开展分析。