Genes regulated by miR-127-3p in glioblastoma cell line. Homo sapiens

Overexpression of miR-127-3p in LN229 glioblastoma cells promotes their migration and invasion in vitro and in vivo in xenograft models. We used microarrays to detail the global programme of gene expression in miR-127-3p overexpression LN229 cells compared with mock overexpression LN229 cells Overall design: MiR-127-3p overexpression LN229 cells and and mock overexpression LN229 cells were cultured in DMEM cell culture media for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the genes regulated by miR-127-3p in glioblastoma cell lines.

在LN229胶质母细胞瘤细胞中过表达miR-127-3p，可在体外实验及异种移植模型（xenograft models）的体内实验中促进该细胞的迁移与侵袭能力。本研究通过微阵列（microarrays）技术，详细解析了相较于空载过表达LN229细胞，miR-127-3p过表达LN229细胞的全基因表达谱。
实验设计：将miR-127-3p过表达LN229细胞与空载过表达LN229细胞培养于DMEM细胞培养基中，用于RNA提取及在Affymetrix微阵列上进行杂交。本研究旨在筛选出胶质母细胞瘤细胞系中受miR-127-3p调控的基因。