IDHwt and IDHmut adult-type diffuse gliomas display distinct alterations in ribosome biogenesis and 2'O-methylation of ribosomal RNA

We simultaneously characterized alterations of both ribosome quality and quantity in high grade adult-type diffuse glioma. Unexpectedly, we uncovered that glioblastomas display the most prominent defects in ribosomal RNA chemical modifications (quality) while astrocytomas and oligodendrogliomas rather exhibit changes in ribosome biogenesis (quantity). Moreover, glioma cell lines exhibit distinct responses to clinically evaluated RNA Pol I inhibitors, CX5461 and BMH-21. This study highlights theranostic opportunities provided by the ribosome field to improve the management of glioma patients. Overall design: ribosomal RNA 2'-O-ribose methylation profile in high grade adult-type glioma

本研究同时表征了成人高级别弥漫性胶质瘤中核糖体质量与数量的改变。出乎意料的是，本研究发现胶质母细胞瘤在核糖体RNA化学修饰（质量层面）存在最为显著的缺陷，而星形细胞瘤与少突胶质细胞瘤则更多表现为核糖体生物发生（数量层面）的改变。此外，胶质瘤细胞系对临床评估阶段的RNA聚合酶I（RNA Pol I）抑制剂CX5461与BMH-21呈现出截然不同的应答反应。本研究凸显了核糖体研究领域为改善胶质瘤患者诊疗管理所提供的治疗诊断学机遇。实验设计：成人高级别胶质瘤中核糖体RNA的2'-O-核糖甲基化谱