Effect of cisplatin + anti-PD1 + anti-CTLA-4 treatment on gene expression of mouse eosinophils in blood of mice with mammary-tumor derived metastasis

Immune checkpoint blockade (ICB) has opened a new era in cancer therapy. Here, we studied the potential role of eosinophils in the response to ICB treatment and chemotherapy combination in mouse models of primary and metastatic breast cancer. As a model of metastatic breast cancer, we used mice with metastasis originating from orthotopically transplanted mammary tumors, which in turn derived from spontaneous mammary tumors of K14cre;Cdh1F/F;Trp53F/F mice. In this study, the gene expression profile of circulating eosinophils sorted from mice with metastatic cancer treated with a combination of cisplatin and anti-PD1 plus anti-CTLA-4 antibodies was analyzed br RNA-sequencing. Overall design: Comparative gene expression profiling analysis of RNA-seq data for mouse eosinophils sorted from blood of mice with metastatic cancer treated with cisplatin + anti-PD1 + anti-CTLA4 antibody (n=7 mice) or with control antibody alone (n=4 mice).

免疫检查点阻断（Immune Checkpoint Blockade, ICB）为癌症治疗开启了全新纪元。本研究旨在探究嗜酸性粒细胞（eosinophils）在原发性与转移性乳腺癌小鼠模型中，对免疫检查点阻断联合化疗的应答潜力。本研究选用的转移性乳腺癌模型，其转移灶源自原位移植的乳腺肿瘤；而该原位移植瘤又来自K14cre;Cdh1F/F;Trp53F/F小鼠的自发性乳腺肿瘤。本研究通过RNA测序（RNA-sequencing），对经顺铂联合抗PD-1及抗CTLA-4抗体治疗的转移性乳腺癌小鼠的循环嗜酸性粒细胞的基因表达谱进行了分析。实验整体设计：针对两组小鼠血液中分选得到的嗜酸性粒细胞的RNA测序数据开展对比基因表达谱分析，两组分别为经顺铂+抗PD-1+抗CTLA-4抗体治疗的转移性乳腺癌小鼠（n=7只），以及仅接受对照抗体处理的对照组小鼠（n=4只）。