Distribution of ApoEnull mice oral microbial samples positive for bacterial genomic DNA by PCR.

aIndicate time points at which oral microbial samples were collected (2, 8, 14, and 16 weeks) following polymicrobial infection for determination of microbial colonization by species specific PCR analysis.bIndicate mice were infected for 8 alternate weeks with P. gingivalis, T. denticola, and T. forsythia. Samples were analyzed using appropriate specific PCR primers with positive and negative controls.cOral microbial samples were collected from sham-infected control mice periodically and examined for P. gingivalis, T. denticola, and T. forsythia using bacteria specific primers and all mice were negative. - Indicate oral microbial samples negative by PCR analysis. Pg-indicate P. gingivalis; Td-indicate T. denticola; and Tf-indicate T. forsythia.

a 标注多微生物感染后口腔微生物样本的采集时间点（第2、8、14和16周），用于通过物种特异性聚合酶链式反应（PCR）分析微生物定植情况。
b 说明小鼠以8个间隔周的频率交替感染牙龈卟啉单胞菌（P. gingivalis）、齿垢密螺旋体（T. denticola）与福赛斯坦纳菌（T. forsythia）；采用适配的特异性PCR引物结合阳性、阴性对照对样本进行分析。
c 定期从假感染对照小鼠中采集口腔微生物样本，使用细菌特异性引物检测牙龈卟啉单胞菌、齿垢密螺旋体与福赛斯坦纳菌，所有小鼠样本均呈阴性。—— 标注经PCR分析为阴性的口腔微生物样本。其中Pg代表牙龈卟啉单胞菌（P. gingivalis），Td代表齿垢密螺旋体（T. denticola），Tf代表福赛斯坦纳菌（T. forsythia）。