Dataset on the changes in the gut microbiome in high-fat diet-fed mice following faecal microbiota transplantation from inulin dosed mice

Inulin has been reported to exert beneficial effects on the prevention and treatment of obesity-related disorders, but its potential mechanisms still need to be further explored. Herein we treated HFD-fed mice with intragastric administration of inulin for 10 weeks and investigated its impacts on the gut microbiota and obesity-related disorders. Subsequently, the faecal microbiota from inulin dosed mice was transferred to HFD-induced obese recipient mice to confirm the causal role of the gut microbiota in the protective effect of inulin on obesity-related disorders. In order to examine the gut microbial changes, we used a 16S rRNA gene sequencing approach to analyze the gut microbiome in feces of mice receiving normal chow (Chow), high-fat diet (HFD), HFD/inulin (INU), faecal microbiota from inulin-treated mice (INUR) and faecal microbiota from HFD-induced obese mice (HFDR). <br>

已有研究表明，菊粉（Inulin）对肥胖相关疾病的预防与治疗具有有益作用，但其潜在作用机制仍有待进一步探究。本研究中，我们对高脂饮食（High-Fat Diet，以下简称HFD）喂养的小鼠进行为期10周的菊粉灌胃处理，并探究其对肠道菌群与肥胖相关疾病的影响。随后，我们将经菊粉处理的小鼠的粪便菌群移植至高脂饮食诱导的肥胖受体小鼠体内，以验证肠道菌群在菊粉发挥抗肥胖相关疾病保护作用中的因果性作用。为检测肠道微生物的组间变化，我们采用16S rRNA基因测序技术，对以下5组小鼠的粪便菌群进行分析：正常饲料组（Chow）、高脂饮食组（HFD）、高脂饮食联合菊粉组（HFD/INU）、菊粉处理小鼠粪便菌群移植组（INUR）以及高脂饮食诱导肥胖小鼠粪便菌群移植组（HFDR）。