Transcriptome sequencing of liver tissue in hepatocyte-specific IRF8-deficient mice (IRF8-cKO) mice and wild type (WT) mice that were subjected to warm ischemia/reperfusion(I/R) injury

Interferon regulatory factor 8 (IRF8), also known as interferon consensus sequence-binding protein, is an essential lineage-specific transcriptional regulator in the IRF family, controls diverse cellular processes and is has been implicated in innate immunity, immune cell differentiation. IRF8 has been shown to have both oncogenic and tumor suppressor activities, in myeloid cells and nonhematopoietic cells. As a transcription factor, other functions which may contribute to its oncogenic and anti tumor potential are not clear. So it is meaningful to study the role of IRF8 in different diseases. Our recent study demonstrated the protective effects of IRF8 on the liver cancer through the regulation of immune response. In addition, studies have shown that IRF8 has protective effects on ischemia-reperfusion injury of heart and brain and we wondered. However, the role of IRF8 in hepatic ischemia-reperfusion injury has not been reported. Overall design: mRNA profile of injured liver tissues were generated by deep sequencing in hepatocyte-specific IRF8-deficient mice(IRF8-cKO) mice and wild type mice(WT) that were subjected to warm ischemia/reperfusion injury (I/R)

干扰素调节因子8（Interferon regulatory factor 8, IRF8），亦称干扰素共识序列结合蛋白（interferon consensus sequence-binding protein），属于IRF家族中关键的谱系特异性转录调控因子，可调控多种细胞生理过程，且已被证实与先天免疫、免疫细胞分化密切相关。IRF8在髓系细胞与非造血细胞中均被发现兼具致癌活性与抑癌活性。作为一类转录因子，其促癌与抗肿瘤潜能背后的其他潜在调控机制尚不明确，因此探究IRF8在不同疾病中的作用具有重要研究价值。我们近期的研究证实，IRF8可通过调控免疫应答发挥对肝癌的保护作用。此外，已有研究表明IRF8对心脑缺血再灌注损伤（ischemia-reperfusion injury, I/R）具有保护效应，我们对此抱有探究兴趣。然而，目前尚未有关于IRF8在肝缺血再灌注损伤中作用的相关报道。本研究的整体实验设计：对经受温热缺血再灌注损伤（warm ischemia/reperfusion injury, I/R）的肝细胞特异性IRF8敲除小鼠（hepatocyte-specific IRF8-deficient mice, IRF8-cKO）与野生型小鼠（wild type mice, WT）的损伤肝组织进行深度测序，以获取其mRNA表达谱。