Strand-specific RNA sequencing of specific brain regions of the zebrafish

Total RNA was isolated from three brain regions (forebrain, midbrain, and hindbrain) of wild type adult zebrafish (Danio rerio). Strand-specific RNA was sequenced after ribosomal depletion by Ribo-Zero gold kit. Long non-coding RNAs (lncRNAs) and mRNAs were detected and differential expression studied.

本研究从野生型成年斑马鱼（Danio rerio）的三个脑区（端脑、中脑、后脑）中提取总RNA。采用Ribo-Zero Gold试剂盒去除核糖体RNA后，对样本开展链特异性RNA测序。本研究检测了长链非编码RNA（long non-coding RNAs，lncRNAs）与信使RNA（messenger RNA，mRNA），并对二者的差异表达情况进行了分析研究。