Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset]

Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles (“SmartFlares”) can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both – SmartFlares and unmodified GNPs – lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles.

定位显微镜已被证明能够以纳米分辨率对金纳米颗粒（GNP）的细胞摄取排列与计数开展系统性研究。本文中，我们发现应用经特殊修饰的RNA靶向金纳米颗粒（“SmartFlares”）可在细胞核周围形成环形GNP排列。透射电子显微镜显示，GNP在细胞内膜结构附近积聚，包括内质网的膜结构。作为原理验证，我们通过γH2AX焦点分析对放射治疗剂量增强效应进行了量化：与未处理的乳腺癌细胞相比，SmartFlares和未修饰GNP的应用均显著提升了剂量沉积，增强因子最高可达1.2倍。SmartFlares的增强效应更为显著。此外，研究表明，照射期间同时施加1特斯拉的磁场对金纳米颗粒的结构及其介导的剂量增强效应均无显著影响。